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Widespread posttranslational modifications include methylation of the ε-amino group, giving methyl- , dimethyl- , and trimethyllysine The latter occurs in calmodulin Other posttranslational modifications at lysine residues embrace acetylation , sumoylation , and ubiquitination Collagen incorporates hydroxylysine , which is derived from lysine by lysyl hydroxylase - Glycosylation of hydroxylysine residues in the endoplasmic reticulum or Golgi apparatus is used to mark certain proteins for secretion from the cell In opsins like rhodopsin and the visible opsins (encoded by the genes OPN1SW , OPN1MW , and OPN1LW ), retinaldehyde kinds a Schiff base with a conserved lysine residue, and interaction of light with the retinylidene group causes signal transduction in colour vision (See visual cycle for details). |
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OBSERVE: After the coupling of the first C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and likewise among the amino groups within the spot areas don't kind bonds with the amino acids. BE AWARE: This avoids the coupling of amino acids in the additional cycles with the membrane as an alternative of the growing peptide chain. Make sure all the purification buffers are at 4 °C and carry out the protein purification in the chilly room to avoid protein denaturation. |
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